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Evident Corporation brightfield microscope ix-81
Brightfield Microscope Ix 81, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ix-81/bx51+microscope/pm39214305-242-6-6
Average 90 stars, based on 1 article reviews
brightfield microscope ix-81 - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Confocal Microscopy:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).

Inverted Microscopy:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).

Fluorescence:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).

Microscopy:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).

Labeling:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).

Software:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).

Spectroscopy:

Article Title: Matrix confinement modulates 3D spheroid sorting and burst-like collective migration
Article Snippet: Confocal microscopy Images were taken using an oil immersion UplanFL N 10 x/1.30 NA (Olympus) objective on an inverted microscope (Olympus IX-81) equipped with an iXON3 EMCCD camera (Andor Technology), National Instrument DAQ-MX controlled laser (Solamere Technology), and a Yokogawa CSU-X1 spinning disk confocal unit.

Article Title: Invadopodia enable cooperative invasion and metastasis of breast cancer cells.
Article Snippet: Samples were imaged on a widefield microscope (IX-81, Olympus) equipped with an LED lamp (Excelitas Technologies), an Orca 16-bit chargecoupled device camera (Hamamatsu), an automated z-drift compensation IX3ZDC (Olympus), an automated stage (Prior Scientific), an environmental chamber (Live Cell Instrument) and using a 10X objective (MPlanFL N 10X, 0.3 NA, Olympus).

Article Title: Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells
Article Snippet: AFM indentation was conducted on a JPK NanoWizard-1 (JPK Instruments) operating in force spectroscopy mode, mounted on an inverted optical microscope (IX-81; Olympus).

Article Title: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Article Snippet: Tissues were imaged in a live-cell chamber (STX Temp & CO 2 Stage Top Incubator, Tokai Hit) using a sCMOS camera (Zyla 4.2, Andor Technology) connected to an inverted fluorescence microscope with a standard GFP filter set (IX-81, Olympus).

Article Title: Particulate Matter-Induced Neurotoxicity: Unveiling the Role of NOX4-Mediated ROS Production and Mitochondrial Dysfunction in Neuronal Apoptosis
Article Snippet: Images of MitoSOX fluorescence were collected within 5 min using 510 nm for excitation and 580 nm emission (IX-81; Olympus, Tokyo, Japan) with a confocal spinning disk (CSU10; Yokogawa, Tokyo, Japan).

Article Title: lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.
Article Snippet: A quantitative digital Image analysis system (Image Pro-plus Version 7.0; Media Cybernetics, Rockville, MD) with digital cameras (Olympus IX-81, Olympus, Tokyo, Japan) was adopted.

Article Title: Ciprofloxacin-Loaded Poly( N -isopropylacrylamide- co -acrylamide)/Polycaprolactone Nanofibers as Dual Thermo- and pH-Responsive Antibacterial Materials.
Article Snippet: Nanofibers are an attractive option in drug release, especially as antibacterial materials.. However, there is no universal antibacterial material and little attention has been devoted to bacteria− nanofiber attachment.. Poly(N-isopropylacrylamide-co-acrylamide) is particularly interesting due to its dual thermoand pH-responsive nature.

Article Title: Long-Term Functional and Cytoarchitectonic Effects of the Systemic Administration of the Histamine H 1 Receptor Antagonist/Inverse Agonist Chlorpheniramine During Gestation in the Rat Offspring Primary Motor Cortex
Article Snippet: Micrographs of M1 were obtained with a charge-coupled device camera (ORCA-Flash 2.8 CCD model C11440; Hamamatsu, Honshu, Japan) coupled to the epifluorescence microscope (Olympus IX-81, Shinjuku, Tokyo, Japan).



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90
Evident Corporation fluoview fv1000 inverted ix 81 confocal microscope
Effects of enhanced S1P turnover and FFA on overall oxidative stress and cytosolic hydrogen peroxide production in insulin-secreting INS1E cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. (A) overall oxidative stress was measured by DCFDA method. (B) cytosolic hydrogen peroxide generation was estimated by expressing the hydrogen peroxide-sensitive fluorescence sensor HyPer-Cyto protein in the cytosolic compartment of cells and evaluation of fluorescence shift by the CellSens software at the Olympus fluorescence <t>microscope</t> (representative pictures from n = 3 individual experiments), Bars: 10 μm. Shown (A) are Means ± SEM from n = 3–6 independent experiments, each condition was measured in triplicates ANOVA followed by Bonferroni, ∗∗ P < 0.01, ∗∗∗ P < 0.001 versus untreated, # P < 0.05, ## P < 0.01, versus INS1E-ctr cells treated in the same way.
Fluoview Fv1000 Inverted Ix 81 Confocal Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of enhanced S1P turnover and FFA on overall oxidative stress and cytosolic hydrogen peroxide production in insulin-secreting INS1E cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. (A) overall oxidative stress was measured by DCFDA method. (B) cytosolic hydrogen peroxide generation was estimated by expressing the hydrogen peroxide-sensitive fluorescence sensor HyPer-Cyto protein in the cytosolic compartment of cells and evaluation of fluorescence shift by the CellSens software at the Olympus fluorescence microscope (representative pictures from n = 3 individual experiments), Bars: 10 μm. Shown (A) are Means ± SEM from n = 3–6 independent experiments, each condition was measured in triplicates ANOVA followed by Bonferroni, ∗∗ P < 0.01, ∗∗∗ P < 0.001 versus untreated, # P < 0.05, ## P < 0.01, versus INS1E-ctr cells treated in the same way.

Journal: Journal of Lipid Research

Article Title: The fate of intracellular S1P regulates lipid droplet turnover and lipotoxicity in pancreatic beta-cells

doi: 10.1016/j.jlr.2024.100587

Figure Lengend Snippet: Effects of enhanced S1P turnover and FFA on overall oxidative stress and cytosolic hydrogen peroxide production in insulin-secreting INS1E cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. (A) overall oxidative stress was measured by DCFDA method. (B) cytosolic hydrogen peroxide generation was estimated by expressing the hydrogen peroxide-sensitive fluorescence sensor HyPer-Cyto protein in the cytosolic compartment of cells and evaluation of fluorescence shift by the CellSens software at the Olympus fluorescence microscope (representative pictures from n = 3 individual experiments), Bars: 10 μm. Shown (A) are Means ± SEM from n = 3–6 independent experiments, each condition was measured in triplicates ANOVA followed by Bonferroni, ∗∗ P < 0.01, ∗∗∗ P < 0.001 versus untreated, # P < 0.05, ## P < 0.01, versus INS1E-ctr cells treated in the same way.

Article Snippet: Cells were cultured for 48 h and afterwards exposed to FFAs for 24 h. Live cell imaging was performed using a CFP-YFP dual filter (excitation, 427 nm and 504 nm; emission, 520 nm) with a Olympus IX 81 inverted microscope system and the CellSens software (Olympus, Hamburg, Germany) for imaging and analysis.

Techniques: Incubation, Expressing, Fluorescence, Software, Microscopy

Effects of enhanced S1P turnover and FFA on peroxisomal and mitochondrial hydrogen peroxide production in insulin-secreting INS1E cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. Peroxisomal hydrogen peroxide generation was estimated by expressing the fluorescence sensor HyPer-Peroxi (A) and mitochondrial by expressing HyPer-Mito (B) sensor proteins and evaluation of fluorescence shift by the CellSens software at the Olympus fluorescence microscope (representative pictures from n = 3 individual experiments). Arrows depict representative cells with higher red HyPer protein fluorescence. Bars: 10 μm.

Journal: Journal of Lipid Research

Article Title: The fate of intracellular S1P regulates lipid droplet turnover and lipotoxicity in pancreatic beta-cells

doi: 10.1016/j.jlr.2024.100587

Figure Lengend Snippet: Effects of enhanced S1P turnover and FFA on peroxisomal and mitochondrial hydrogen peroxide production in insulin-secreting INS1E cells. INS1E cells were incubated in the absence or presence of PA or OA (500 μM of each) for 24 h. Peroxisomal hydrogen peroxide generation was estimated by expressing the fluorescence sensor HyPer-Peroxi (A) and mitochondrial by expressing HyPer-Mito (B) sensor proteins and evaluation of fluorescence shift by the CellSens software at the Olympus fluorescence microscope (representative pictures from n = 3 individual experiments). Arrows depict representative cells with higher red HyPer protein fluorescence. Bars: 10 μm.

Article Snippet: Cells were cultured for 48 h and afterwards exposed to FFAs for 24 h. Live cell imaging was performed using a CFP-YFP dual filter (excitation, 427 nm and 504 nm; emission, 520 nm) with a Olympus IX 81 inverted microscope system and the CellSens software (Olympus, Hamburg, Germany) for imaging and analysis.

Techniques: Incubation, Expressing, Fluorescence, Software, Microscopy